intercellular trafficking of vp22, a herpes simplex virus type 1 tegument protein
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abstract
the herpes simplex virus type 1 (hsv-1) tegument protein, vp22 has been reported to have the property of intercellular transport. the previous studies have shown that following expression of a fusion protein containing vp22 it spreads to every cell in a monolayer and concentrates in the nucleus. in spite of these reports, some studies have shown that vp22 trafficking and its nucleus accumulation is an artifact and no improvement in translocation of proteins fused to vp22 has been detected. methods: to better understand about vp22 translocation, vp22-gfp (green fluorescent protein) vector was constructed and its nuclear accumulation, transportation to the nontransfected cells and translocation between different cell types were studied by fluorescent microscope. results: vp22-fusion protein was detected in nontransfected cells which in some of them the fusion protein was shown in nucleus. conclusion: the results demonstrated that vp22 can easily transport between different cells but nuclear accumulation of the protein is not common in all of the recipient cells.
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Intercellular Trafficking of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
The herpes simplex virus type 1 (HSV-1) tegument protein, VP22 has been reported to have the property of intercellular transport. The previous studies have shown that following expression of a fusion protein containing VP22 it spreads to every cell in a monolayer and concentrates in the nucleus. In spite of these reports, some studies have shown that VP22 trafficking and its nucleus accumulatio...
full textIntercellular trafficking of VP22, a herpes simplex virus type 1 tegument protein.
BACKGROUND The herpes simplex virus type 1 (HSV-1) tegument protein, VP22 has been reported to have the property of intercellular transport. The previous studies have shown that following expression of a fusion protein containing VP22; it spreads to every cell in a monolayer and concentrates in the nucleus. In spite of these reports, some studies have shown that VP22 trafficking and its nucleus...
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Tegument proteins of herpes simplex virus type 1 (HSV-1) are hypothesized to contain the functional information required for the budding or envelopment process proposed to occur at cytoplasmic compartments of the host cell. One of the most abundant tegument proteins of HSV-1 is the U(L)49 gene product, VP22, a 38-kDa protein of unknown function. To study its subcellular localization, a VP22-gre...
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The alphaherpesvirus tegument protein VP22 has been characterized with multiple traits including microtubule reorganization, nuclear localization, and nonclassical intercellular trafficking. However, all these data were derived from studies using herpes simplex virus type 1 (HSV-1) and may not apply to VP22 homologs of other alphaherpesviruses. We compared subcellular attributes of HSV-1 VP22 (...
full textThe Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
BACKGROUND Genetic modifications can improve the therapeutic efficacy of mesenchymal stem cell (MSC) transplantation in myocardial infarction. However, so far, the efficiency of MSC modification is very low. Seeking for a more efficient way of MSC modification, we investigated the possibility of employing the intercellular trafficking capacity of the herpes simplex virus type-1 tegument protein...
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Journal title:
iranian biomedical journalجلد ۱۱، شماره ۱، صفحات ۵۳-۵۷
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